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Unstable retention times
Retention times (RTs) must be stable across multiple injections. Retention time shift Reproducible retention time Retention time fluctuations during a measurement are usually caused by the LC system. The most common causes are 1. Pump malfunctions, ...
Why the extracts did not go through the filters or the volumes in the capture plate are low/inconsistent?
Inconsistent or lack of volume in the filter plate may occur for several reasons. Below are some suggestions: Water or another solvent was used for making the extraction solvent instead of Methanol. Perform the following steps: Transfer the extracts ...
Change plate layout after kit run
Change plate run after kit run. Please follow the instructions in the pdf attachment.
Why do extracts appear cloudy?
For AbsoluteIDQ® p180, p400 HR and MxP® Quant 500 kits: When adding water to the methanolic sample extracts to make the LC plate, cloudiness can occur due to residues of pyridine and phenyl isothiocyanate from the derivatization solution. If that ...
How long will my kits remain stable when stored?
Expiration date for Quant 500 and Quant 500 XL kits: Storage at -20 °C: 3 months after shipping date Storage at -80 °C: 12 months after shipping date Expiration date for p180, p400 HR, Quant HR Xpress and Bile Acids kits: Storage at -20 °C: 6 months ...