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Why my metabolite concentrations deviate from predicted concentrations?
Metabolite intensities (peak areas) depend on several factors, such as ion suppression effects, caused by the sample’s matrix, metabolite dilution or extraction effects, e.g. the metabolite extraction efficiency from a cell pellet, or the ratio of ...What are the common sources of fatty acids contamination in the LC system?
Fatty acids (FA) are substances, which are common contaminations in LC-MS systems and they “like to remain” in the system. FA are part of the most biological samples and can be easily broguht into the system. e.g., solvent bottles. Even after a ...What happens if I use the kit after it expires?
All kit components requiring a refrigeration are subject to expiration (light blue box). These are the Vial Box and the Kit Plate. After passing the expiration date, the kit can still be used. However, the proper performance cannot be guaranteed. ...Missing concentration values in Results
Whenever a concentration cannot be calculated, a specific status is shown in WebIDQ > Results. Due to analytical reasons, concentration values may not be available, and will be represented by these statuses: NA Empty values are replaced with NA NaN ...What kind of plasma anticoagulants are compatible with biocartes kits?
biocrates kits were validated with EDTA and heparin plasma only. Citrate plasma may also be used, but performance may be reduced. The most important aspect is to ensure that all plasma samples within one study are collected in the same way and using ...
Popular Articles
Why the extracts did not go through the filters or the volumes in the capture plate are low/inconsistent?
Inconsistent or lack of volume in the filter plate may occur for several reasons. Below are some suggestions: Water or another solvent was used for making the extraction solvent instead of Methanol. Perform the following steps: Transfer the extracts ...Change plate layout after kit run
Change plate run after kit run. Please follow the instructions in the pdf attachment.How long will my kits remain stable when stored?
Expiration date for Quant 500 and Quant 500 XL kits: Storage at -20 °C: 3 months after shipping date Storage at -80 °C: 12 months after shipping date Expiration date for p180, p400 HR, Quant HR Xpress and Bile Acids kits: Storage at -20 °C: 6 months ...What other laboratory equipment and chemicals are required to run the kits that is not provided by biocrates?
While the biocrates kits come with all the standards and quality controls necessary, some other additional chemicals and solvents and lab equipments are required to successfully prepare and run a plate. Please download the respective kit requirements ...FIA chromatograms show signal suppressions
If the FIA chromatograms show signal suppressions, it is most likely either caused by the injection system or by an issue at the ion source. To solve this issue, check the following parts at the LC-MS system: Inspect the ESI electrode and its ...