No, the QC samples are not the same for PITC and NPH. Separate sets of QC samples are provided specifically for each part of the kit. Please refer to the respective kit user manual.
If the QC accuracies are consistent for all measured analytes, but differ significantly from the expected values (i.e. all metabolites for any particular QC sample are at 50% accuracy), the following may have occurred: Pipetting issue: More or less ...
If a pressure manifold is to be used with the MxP Quant 1000 kit, a special adapter is required for drying steps, please see the Product number: 20981. Do not use the adapter for pressure manifold for the elution of extracts.
No, the Calibration Standards are not the same for PITC and NPH. Separate sets of Calibration Standards are provided specifically for each part of the kit.
If you wish to add more sample volume on a filter of the test plate, please note that extra drying steps are required to ensure proper results. Please see the table below. Kit part Sample volume Standard Maximum NPH 20 µL 30 µL PITC 10 µL dry after ...