Does the whole kit plate need to be prepared at once?

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• Why the extracts did not go through the filters or the volumes in the capture plate are low/inconsistent?

  Inconsistent or lack of volume in the filter plate may occur for several reasons. Below are some suggestions: Water or another solvent was used for making the extraction solvent instead of Methanol. Perform the following steps: Transfer the extracts ...

• How long will my kits remain stable when stored?

  Expiration date for Quant 500 and Quant 500 XL kits: Storage at -20 °C: 3 months after shipping date Storage at -80 °C: 12 months after shipping date Expiration date for p180, p400 HR, Quant HR Xpress and Bile Acids kits: Storage at -20 °C: 6 months ...

• What other laboratory equipment and chemicals are required to run the kits that is not provided by biocrates?

  While the biocrates kits come with all the standards and quality controls necessary, some other additional chemicals and solvents and lab equipments are required to successfully prepare and run a plate. Please download the respective kit requirements ...

• Why are there signals in the blank or in the zero samples, why are analytes in the zero samples above LOD?

  The blank well A1 does not contain any analytes or internal standards. The zero samples only contain internal standards, but no analytes. If there are still peaks the following may have occurred: A sample or internal standard mix was accidentally ...

• Why are QC accuracies consistently low or high for all analytes?

  If the QC accuracies are consistent for all measured analytes, but differ significantly from the expected values (i.e. all metabolites for any particular QC sample are at 50% accuracy), the following may have occurred: 1. Pipetting issue: More or ...