How is LOD calculated?

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  The blank well A1 does not contain any analytes or internal standards. The zero samples only contain internal standards, but no analytes. If there are still peaks the following may have occurred: A sample or internal standard mix was accidentally ...

• Why are QC accuracies consistently low or high for all analytes?

  If the QC accuracies are consistent for all measured analytes, but differ significantly from the expected values (i.e. all metabolites for any particular QC sample are at 50% accuracy), the following may have occurred: 1. Pipetting issue: More or ...

• Why the extracts did not go through the filters or the volumes in the capture plate are low/inconsistent?

  Inconsistent or lack of volume in the filter plate may occur for several reasons. Below are some suggestions: Water or another solvent was used for making the extraction solvent instead of Methanol. Perform the following steps: Transfer the extracts ...