How to choose correct RT when there are multiple peaks?
WebIDQ contains a tool Auto peak picking for defaults for automatically determine the retention time of each metabolite. It will set the same default retention time ranges for
each sample based on the quality controls (QCs). After applying Auto peak picking, if you are uncertain, follow the respective kit user
manual and check the example RT excel sheet to estimate the retention times for
your instrument.
For example metabolites such as "AABA"
which have multiple peaks may be integrated incorrectly.
The trick is here to check the
peaks just eluting before and after the AABA in the example RT excel sheet.
By using the excel RT sheet, estimated retention time for AABA can be
calculated by checking the peaks which are eluting before and after the AABA.
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