How to use metabolism indicators (MetaboINDICATORS) in statistical procedures?
Metabolism
indicators shall help to get started with the kit data interpretations. Each
calculated metabolism indicator serves as an added value to the results data
set.
- Detailed information to each metabolism indicator is
provided in the kit specific document biocrates-KIT
Name list of metabolite sums and ratios (v#-YYYY), column “literature”,
available on the Kit files, folder “Documents and
Notes\MetaboINDICATOR”.
- As a simple rule,
metabolism indicators should be incorporated in univariate analyses like
statistical comparisons and correlation analysis just like additional
metabolite features.
- In multivariate analyses
like PCA and PLS-DA, however, metabolism indicators should not be included,
because the information they contain on the metabolome is already present in
the form of the metabolites they are calculated from, so adding them would add
more weight to the metabolites these metabolites and skew the analysis.