Maximum unknown sample volumes for MxP Quant 1000 kit?

If you wish to add more sample volume on a filter of the test plate, please note that extra drying steps are required to ensure proper results. Please see the table below.

Kit part	Sample volume
	Standard	Maximum
NPH	20 µL	30 µL
PITC	10 µL	dry after each 20 µL
Lipid	10 µL	dry after each 20 µL

Information for PITC and Lipid parts

After adding a sample volume of 20 µL to a filter of the test plate, include an additional drying step of 30 min. In theory, there is no maximum sample volume. However, for sample volumes larger than 30 µL, matrix effects may become predominant.

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• Pressure manifold with MxP Quant 1000 kit

  If a pressure manifold is to be used with the MxP Quant 1000 kit, a special adapter is required for drying steps, please see the Product number: 20981. Do not use the adapter for pressure manifold for the elution of extracts.

• Are the Calibration Standrads the same for both PITC and NPH parts of the MxQuant kit?

  No, the Calibration Standards are not the same for PITC and NPH. Separate sets of Calibration Standards are provided specifically for each part of the kit.

• Are the QC samples the same for both PITC and NPH parts of the MxQuant kit?

  No, the QC samples are not the same for PITC and NPH. Separate sets of QC samples are provided specifically for each part of the kit. Please refer to the respective kit user manual.

• What is the runtime per sample and kit?

  Run time per sample / min Run time per kit / h

• Can I use speed vac for drying the test plate?

  Officially biocrates does not recommend using a speed vac instead of a nitrogen drying system. The use of a speed vac for kit preparation procedures was never tested by biocrates. However and for sure, a speed vac can be used. If a speed vac is used, ...