What other laboratory equipment and chemicals are required to run the kits that is not provided by biocrates?
While the biocrates kits come with all the standards and quality controls necessary, some other additional chemicals and solvents and lab equipments are required to successfully prepare and run a plate.
Please download the respective kit requirements document for the product you want to buy. The full mass spectrometry system compatibility list can be found here.
Please contact biocrates support for a technical session or further information.
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How long will my kits remain stable when stored?
Expiration date for Quant 500 and Quant 500 XL kits: Storage at -20 °C: 3 months after shipping date Storage at -80 °C: 12 months after shipping date Expiration date for p180, p400 HR, Quant HR Xpress and Bile Acids kits: Storage at -20 °C: 6 months ...Why the extracts did not go through the filters or the volumes in the capture plate are low/inconsistent?
Inconsistent or lack of volume in the filter plate may occur for several reasons. Below are some suggestions: Water or another solvent was used for making the extraction solvent instead of Methanol. Perform the following steps: Transfer the extracts ...Why do extracts appear cloudy?
For AbsoluteIDQ® p180, p400 HR and MxP® Quant 500 kits: When adding water to the methanolic sample extracts to make the LC plate, cloudiness can occur due to residues of pyridine and phenyl isothiocyanate from the derivatization solution. If that ...Does the whole kit plate need to be prepared at once?
A kit must be prepared all at once, as it is sealed under nitrogen to prevent degradation. Kits are for single use only.How are lipids reported?
The lipids are annotated according to head group, total number of carbons in the chains, and total number of double bonds in the chains. For example, PC(16:1/20:5) becomes PC(36:6). The lipid analysis in the p180, p400 HR and MxP Quant 500 kits does ...