Mass spectrometer
How should the LC-MS system be maintained for optimal performance?
Important cleaning notes: Frequent cleaning actions and rinsing methods are strongly recommended to minimize the risk of instrument contaminations or performance loss. For optimal time-efficiency, clean and rinse the instruments routinely between the ...
FIA signal is eluting quite late
A late-eluting FIA signal indicates an increased dead volume between the autosampler and the ion source, which can impact chromatographic performance. To address this issue, consider the following: Capillary Length: How long is the capillary between ...
How to choose correct RT when there are multiple peaks?
WebIDQ includes an Auto peak picking tool that automatically determines the retention time for each metabolite. It assigns the same default retention time ranges for all samples based on quality controls (QCs). After applying Auto peak picking, if ...
Combined wiff files or multiple injections in one wiff file
What happens when you import the data in WebIDQ, the wiff files are converted into .mzML data files. Since mzML doesn’t support combined spectra, they are split into two separate files. WebIDQ should always take the most recent file imported, so this ...
What should FIA peaks look like and how can the peak shape be improved?
The best peak shapes are obtained by avoiding unnecessary dead volumes (caused by valves or connection parts) and connecting the injector directly to the mass spectrometer. For this, red PEEK tubing is recommended. The FIA peaks must be located ...
What could cause a loss in sensitivity or absence of peaks?
Please be aware that performance-related issues that cannot be resolved by instrument cleaning are not within biocrates’ responsibility and must be handled by the user or the LC-MS engineer. Based on our experience, however, we are happy to share ...