Results
How to use metabolism indicators (MetaboINDICATORS) in statistical procedures?
General use of metabolism indicators Metabolism indicators shall help to get started with the kit data interpretations. Each calculated metabolism indicator serves as an added value to the results data set. Detailed information to each metabolism ...
Why my metabolite concentrations deviate from predicted concentrations?
Metabolite intensities (peak areas) depend on several factors, such as ion suppression effects, caused by the sample’s matrix, metabolite dilution or extraction effects, e.g. the metabolite extraction efficiency from a cell pellet, or the ratio of ...
Missing concentration values in Results
Whenever a concentration cannot be calculated, a specific status is shown in WebIDQ > Results. Due to analytical reasons, concentration values may not be available, and will be represented by these statuses: NA Empty values are replaced with NA NaN ...
Why are QC accuracies consistently low or high for all analytes?
If the QC accuracies are consistent for all measured analytes, but differ significantly from the expected values (i.e. all metabolites for any particular QC sample are at 50% accuracy), the following may have occurred: 1. Pipetting issue: More or ...
How are lipids reported?
The lipids are annotated according to head group, total number of carbons in the chains, and total number of double bonds in the chains. For example, PC(16:1/20:5) becomes PC(36:6). The lipid analysis in the p180, p400 HR and MxP Quant 500 kits does ...
How is LOD calculated?
The limit of detection (LOD) for each metabolite within a kit run is calculated from the concentrations in the zero samples based on either the standard deviation (StdDev-based) or median (Median-based). StdDev-based: LOD is calculated as median ...