Quant 1000 kit
Is the FIA additive solvent the same for both the Quant1000 and Quant500 XL kits?
Yes, the FIA additive solvent is same for both the Quant1000 and Quant500 XL kits.
Are separate QC samples provided for the LxQuant part of the Quant1000 kit?
Yes, a separate set of QC samples is provided for the LxQuant part of the Quant1000 kit.
Are the Calibration Standrads the same for both PITC and NPH parts of the MxQuant kit?
No, the Calibration Standards are not the same for PITC and NPH. Separate sets of Calibration Standards are provided specifically for each part of the kit.
Are the QC samples the same for both PITC and NPH parts of the MxQuant kit?
No, the QC samples are not the same for PITC and NPH. Separate sets of QC samples are provided specifically for each part of the kit. Please refer to the respective kit user manual.
Why are QC accuracies consistently low or high for all analytes?
If the QC accuracies are consistent for all measured analytes, but differ significantly from the expected values (i.e. all metabolites for any particular QC sample are at 50% accuracy), the following may have occurred: Pipetting issue: More or less ...
Can I use speed vac for drying the test plate?
Officially biocrates does not recommend using a speed vac instead of a nitrogen drying system. The use of a speed vac for kit preparation procedures was never tested by biocrates. However and for sure, a speed vac can be used. If a speed vac is used, ...
Maximum unknown sample volumes for MxP Quant 1000 kit?
If you wish to add more sample volume on a filter of the test plate, please note that extra drying steps are required to ensure proper results. Please see the table below. Kit part Sample volume Standard Maximum NPH 20 µL 30 µL PITC 10 µL dry after ...
Pressure manifold with MxP Quant 1000 kit
If a pressure manifold is to be used with the MxP Quant 1000 kit, a special adapter is required for drying steps, please see the Product number: 20981. Do not use the adapter for pressure manifold for the elution of extracts.
What happens if I use the kit after it expires?
All kit components requiring a refrigeration are subject to expiration (light blue box). These are the Vial Box and the Kit Plate. After passing the expiration date, the kit can still be used. However, the proper performance cannot be guaranteed. ...
What kind of plasma anticoagulants are compatible with biocartes kits?
biocrates kits were validated with EDTA and heparin plasma only. Citrate plasma may also be used, but performance may be reduced. The most important aspect is to ensure that all plasma samples within one study are collected in the same way and using ...
What kind of samples can be measured with the kits?
biocrates kits have been specifically validated for use with plasma and serum. Feasibility tests have been carried out with other sample matrices and protocols are available (see below). Basically, any samples containing endogenous metabolites can be ...
What is the runtime per sample and kit?
Run time per sample / min Run time per kit / h
Does the whole kit plate need to be prepared at once?
A kit must be prepared all at once, as it is sealed under nitrogen to prevent degradation. Kits are for single use only.
What other laboratory equipment and chemicals are required to run the kits that is not provided by biocrates?
While the biocrates kits come with all the standards and quality controls necessary, some other additional chemicals and solvents and lab equipments are required to successfully prepare and run a plate. Please download the respective kit requirements ...
Why are there signals in the blank or in the zero samples, why are analytes in the zero samples above LOD?
The blank well A1 does not contain any analytes or internal standards. The zero samples only contain internal standards, but no analytes. If there are still peaks the following may have occurred: A sample or internal standard mix was accidentally ...
How long will my kits remain stable when stored?
Expiration date for Quant 500 and Quant 500 XL kits: Storage at -20 °C: 3 months after shipping date Storage at -80 °C: 12 months after shipping date Expiration date for p180, p400 HR, Quant HR Xpress and Bile Acids kits: Storage at -20 °C: 6 months ...
Why the extracts did not go through the filters or the volumes in the capture plate are low/inconsistent?
Inconsistent or lack of volume in the filter plate may occur for several reasons. Below are some suggestions: Water or another solvent was used for making the extraction solvent instead of Methanol. Perform the following steps: Transfer the extracts ...
Why do extracts appear cloudy?
For AbsoluteIDQ® p180, p400 HR and MxP® Quant 500 kits: When adding water to the methanolic sample extracts to make the LC plate, cloudiness can occur due to residues of pyridine and phenyl isothiocyanate from the derivatization solution. If that ...